Archive | Protocols

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4 Resources for Zebrafish Research

Posted on 26 December 2010 by Christine

Zebrafish are increasing in popularity in countless research areas. Having made its debut in developmental biology, Danio rerio is swimming through research areas spanning from behavioral neuroscience to cardiac or oncological studies. There are few disciplines I can think of where zebrafish have not yet been used, but with the recent surge in zebrafish research there is also a need for good literature on the topic. Here is a brief list of useful resources for zebrafish research:

The Zebrafish Book

Already in its 5th edition, the Zebrafish Book by Westerfield (2007) is certainly not a new kid on the block, but it is by far the most basic and necessary resource for anyone venturing into zebrafish research. You can either purchase a print version of the book, but you can also access all of the content in the 4th edition of the book online (for free!).
The Zebrafish Book covers general methods for zebrafish care, breeding, embryonic and larval culture, cellular and molecular methods, and protocols.

ZFIN Protocol Wiki

ZFIN has built a Wiki with protocols for zebrafish research. Whether you are using Danio rerio for gene cloning, protein analysis, or microscopic observations… ZFIN has a protocol for it.
As it is a wiki, users can load protocols to the site as well. When using protocols, it is worth keeping in mind that although it being a useful resource it is not peer reviewed. Some of the protocols are based on peer reviewed papers.

ZFIN model organism database

ZFIN is a phenomenal database for all things zebrafish. ZFIN has a database with zebrafish lines and also a page with job listings for people in the field.

Zebrafish Neurobehavioral Protocols

Zebrafish are relatively new in behavioral neuroscience. As a consequence, standardized protocols are still in being developed by the different groups in this field. There is a remarkable need for good protocols, and this book covers a number of protocols that have successfully been used in previous studies.

danio rerio zebrafish resourcesThis list is by no means comprehensive, if you know of some other great resources for beginners in zebrafish research, or for those who have used this model organism for decades, feel free to share it in the comments!

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JoVE Zebrafish protocol alert: Zebrafish Brain Ventricle Injection

Posted on 27 August 2010 by Christine

Since I have last posted, there are some new video articles on JoVE involving zebrafish research. A team at MIT published a video protocol showing how to do injections in the zebrafish brain ventricle. This can aid in the study of ventricle formation and morphology. When studying the effect of teratogens, it could also be interesting to look at brain morphology using this technique. Here are some images shown on JoVE from this protocol.

zebrafish brain morphology research

View the video protocol on JoVE here.

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Never regard your study as a duty, but as the enviable opportunity to learn to know the liberating influence of beauty in the realm of spirit for your own personal joy and to the profit of the community to which your later work belongs. ~ Albert Einstein

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